Week 1: pGLO Transformation

I’m a bit late posting this, as week one was 2 weeks ago now (September 15-21, 2019). Week one in lab I was introduced to doing DNA transformations. On the 17th, we made the media solution that would be poured into 4 plates. On Friday, we were paired up with someone, and each pair got 4 plates that we poured the LB solution in. 2 plates had -pGLO. One of the -pGLO plates had ampicillin in it. Then the other 2 plates had +pGLO and ampicillin. One of these plates also had arabinose. We also learned about pipettes this week and how to use them. On Saturday we spread the E. coli onto the LB solution so it could grow by Tuesday. 

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